Fig. 4. (A ) Typical raw traces of mitochondrial flavoprotein fluorescence MFF in repeated isoflurane (ISO) exposure following a 45-min interval. (B ) Mean (± SD) MFF in repeated and long-interval isoflurane exposures. Data were analyzed at each end of isoflurane exposures and washout (baseline, first isoflurane exposure [ISO-1], washout [Wash], and second isoflurane exposure [ISO-2]). Isoflurane concentrations were 1.7 ± 0.2 mm (high) and 0.8 ± 0.1 mm (low). *P < 0.05 versus  baseline; #P < 0.05 high versus  low isoflurane concentration; +P < 0.05 for first or second isoflurane exposure versus  immediately preceding baseline or washout, as determined by Scheffé test after analysis of variance for repeated measures. a.u. = arbitrary units.