Fig. 2. (A ) Typical raw traces of mitochondrial flavoprotein fluorescence (MFF) in repeated isoflurane (ISO) exposures. (B ) Mean (± SD) MFF in repeated isoflurane exposures. Data were analyzed at the end of each isoflurane exposure and washout (baseline, first isoflurane exposure [ISO-1], first washout [Wash-1], second isoflurane exposure [ISO-2], second washout [Wash-2], and third isoflurane exposure [ISO-3]). Concentrations of isoflurane were 1.5 ± 0.3 mm (high) and 0.8 ± 0.3 mm (low). *P < 0.05 versus  baseline; #P < 0.05 high versus  low isoflurane concentration; +P < 0.05 for first, second, or third isoflurane exposure versus  immediately preceding baseline or washout, as determined by Scheffé test after analysis of variance for repeated measures. a.u. = arbitrary units.