Fig. 8. Effect of halothane on tumor necrosis factor-α (TNF-α) mRNA expression in alveolar type II cells. (A ) Southern-blot analysis of TNF-α cDNA after reverse-transcription polymerase chain reaction. Results of one of four representative experiments. (B ) TNF-α expression in alveolar type II cells expressed as TNF-α/S14 ratio. Results are expressed as mean ± SD; ‡P < 0.001 versus  air. bp = base pair; C = control group; MW = molecular weight.