Fig. 5. The effect of thiopental on nuclear factor κB (NF-κB) activation in primary CD3+T lymphocytes, isolated from the blood of healthy donors, after stimulation with tumor necrosis factor α (TNF-α). The cells were incubated with thiopental in vitro for 1 h at the concentrations indicated (lanes 3–6) and subsequently stimulated with 20 U/ml TNF-α for 1 h (lanes 2, 5, and 6) or the respective volumes of ppH2O as vehicle control. Filled arrowhead = position of NF-κB DNA complexes; open circle = a nonspecific activity binding to the probe; open arrowhead = unbound oligonucleotide. The results are representative of six independent experiments.