Fig. 2. Tetracaine-induced changes in surface morphology of PC12 cells. Cell morphology was viewed in a JSM-U3 scanning electron microscope. (A ) Normally cultured PC12 cells have a variable number of microvilli. (B ) After treatment with 1 mm tetracaine for 4 h, some blebs appear on cells and microvilli vanish (B ). (C ) After treatment for 8 h, some cells are developing buds. (Magnification ×3,500.)