Fig. 1. Examples of propidium iodide fluorescence images from control, oxygen–glucose deprivation (“ischemia”), and oxygen–glucose deprivation plus isoflurane-treated hippocampal slice cultures. Images were acquired 3 days after the simulated ischemia. Bright areas indicate propidium iodide fluorescence (dead neurons). Outlines show CA1, CA3, and dentate cell body regions identified by standard and fluorescent microscopy used for serial analysis of cell death.