Figure 2. Endothelial cells were cultured in 96-well plates and incubated with or without 1 mM H2O2for 10 min. Polymorphonuclear leukocytes (PMNs) were stimulated with or without 10 nM N-formyl-methionyl-leucyl-phenylalanine. In one set of experiments, only human umbilical vein endothelial cells (HUVECs) were pretreated with 1 minimum alveolar concentration (MAC) isoflurane (upper) or sevoflurane (lower) for 40 min, respectively; in another set of experiments, only PMNs were pretreated with 1 MAC of isoflurane or sevoflurane for 40 min. Results (adherent PMNs/well) are shown as the mean +/- SD (n = 5 different cultures and PMN batches for each condition, with 10 wells averaged per experiment). *P < 0.05 compared with baseline values (without stimulation);[dagger] P < 0.05 compared with control after the same stimulation step.