Figure 3. Effects of tert-butyl hydroperoxide (t-BOOH) and propofol on the intracellular concentration of glutathione. Astrocytes were incubated in serum-free minimum essential medium for 3 h, and either 40 [micro sign]M propofol or 0.02% Intralipid was added to the medium for the final 1 h. Then the cells were incubated for an additional 1 h in transport medium containing propofol (or Intralipid) with or without 1 mM t-BOOH. Finally, the cells were washed with transport medium and assayed for glutathione. Plotted are the mean +/- SEM values for four independent experiments. *P < 0.05 for the effect of t-BOOH compared with aqueous vehicle (control).