Figure 2. Propofol protects glutamate transport from oxidative stress. Astrocytes were incubated in serum-free minimum essential medium for 3 h and either 8–40 [micro sign]M propofol or 0.02% Intralipid was added to the medium for the final 1 h. Then the cells were incubated for an additional 1 h in transport medium containing propofol (or Intralipid) with or without 1 mM tert-butyl hydroperoxide (t-BOOH). Finally, the cells were washed with transport medium, and glutamate uptake was measured in 1-min transport assays. Plotted are the mean +/- SEM values for Na+-dependentuptake rates from three to six independent experiments. *P < 0.05 for the effect of t-BOOH compared with aqueous vehicle (control).