Fig. 2. Saturation curve of [3H]norepinephrine uptake. (A ) Cells were incubated with or without 100 μm propofol, thiamylal, or diazepam, and in the presence of various concentrations (1–30 μm) of [3H]norepinephrine at 37°C for 15 min. The results are the mean (±SD) of four separate experiments conducted in duplicate. (B ) Eadie-Hofstee analysis of [3H]norepinephrine uptake. The data were obtained from fig. 2A. V/S = velocity/substrate.