Fig. 2. Change in the intracellular calcium concentration ([Ca2+]i) induced by thrombin at 0.01 U/ml. Samples were pretreated with halothane, isoflurane, or sevoflurane for 5 min. Concentrations of each anesthetic agent were 0.5, 1.0, and 1.5 mM, respectively. The ordinate represents the R340:380 value, indicative of [Ca2+]i; the common abscissa represents time.