Fig. 2. Kainate-activated currents in acutely isolated hippocampal pyramidal neurons from wild-type (+/+) and mutant (−/−) mice. ( A ) Representative kainate-evoked currents at subsaturating (30, 100, and 300 μM) and saturating (1,000 μM) concentrations are shown. ( B ) Concentration–response curves for kainate-evoked currents in (+/+) neurons ( squares ), and (−/−) neurons ( triangles ) were constructed by normalizing to the maximal response and fitting the data points using the modified Michaelis-Menton equation. The concentrations that activated 50% of the maximal current (EC50) and Hill coefficients (nH) were:(+/+) EC50, 226 ± 23 μM (n = 15);(−/−) EC50, 136 ± 7 μM (n = 17);(+/+) nH, 2.06 ± 0.13; and (−/−) nH, 1.69 ± 0.04.