Figure 1. Experimental protocols for the study. Baseline indicates a period of no experimental intervention. All rabbits were subjected to 30 min of coronary occlusion followed by 3 h of reperfusion. The seven groups differed in the pretreatment regimen used. No pretreatment was given in the control group. In the ischemia-preconditioned group, rabbits were subjected to 5 min of coronary occlusion followed by 15 min of reperfusion, before the 30-min period of sustained ischemia. In the isoflurane (ISO) group, 15 min of isoflurane pretreatment at a 1.1% end-tidal concentration was followed by a 15-min washout period. In the isoflurane-plus-glyburide group, 0.33 mg/kg glyburide was administered intravenously 55 min before the prolonged occlusion (and 15 min before isoflurane pretreatment). In the isoflurane plus 8-(p-sulfophenyl)-theophylline (SPT) group, pretreatment was with 7.5 mg/kg intravenous SPT 55 min before prolonged occlusion (15 min before isoflurane pretreatment). In the glyburide-control group, animals were given 0.33 mg/kg glyburide but no isoflurane. In the SPT-control group, animals were given 7.5 mg/kg SPT but no isoflurane. All rabbits that did not receive glyburide were given a control injection of glyburide vehicle. At the end of the 3-h reperfusion period, infarct size and area at risk were measured.