Figure 3. Normalized responses of TASK cRNA-injected oocytes to local anesthetics. (A) Local anesthetic concentration of 10 [micro sign]M. (B) The local anesthetic concentration of 100 [micro sign]M. (C) The Local anesthetic concentration of 1 mM. Studies were performed under a two-electrode voltage clamp in frog Ringer's solution at pH 7.6. The normalized response is defined as current measured for the -80-mV to +40-mV pulse during the treatment condition compared with control. Data are expressed as the mean +/− SD. The numbers in parentheses above the error bars refer to the number of oocytes studied. Local anesthetics are ordered by their octanol:buffer distribution coefficients (Q, increasing from left to right).