Figure 6. Immunoblot detection of thionoacyl halide protein adducts of mercapturates of compound A in rat kidney cytosol incubations. (A) Rat kidney cytosol was incubated for 16 h at 37 [degree sign]C without (lane 1), or with mercapturates of compound A (lane 2). The reactions mixtures were immunoblotted (200 [micro sign]g/lane) at a 1:1,000 dilution of N-TFFMP-GABA-RSA antisera (lanes 1 and 2), or with antisera that had been preincubated with 0.01 mM (lane 3), 0.1 mM (lane 4), or 1.0 mM (lane 5) N-TFFMP-GABA. (B) Rat kidney cytosol was incubated for 16 h at 37 [degree sign]C without (lane 1) or with mercapturates of compound A before (lane 2) or after preincubation with 1.0 mM (lane 3) or 10 mM (lane 4) aminooxyacetic acid. The reaction mixtures were immunoblotted (200 [micro sign]g/lane) with a 1:1,000 dilution of N-TFFMP-GABA antisera.