Figure 2. Kidneys from rats given (A) saline;(B) 500 [micro sign]mol/kg compound 3; or (C) 250 [micro sign]mol/kg compound 5. (A) Control: juxtamedullary region: note the proximal convoluted tubules (P) with abundant brush border. Bar = 50 [micro sign]m. (B) Juxtamedullary region with proximal convoluted tubular epithelium (P) demonstrating focal loss of brush border with excessive intracellular lipid accumulation and occasional pyknotic nucleus (arrow head). Toxicity score of 0.5, PCT. Bar = 50 [micro sign]m. (C) Proximal convoluted tubules (P) in the juxtamedullary region with frank necrosis of the tubular epithelial cell, as indicated by loss of cellular brush border, cytoplasmic basophilia, and loss of nuclei. The necrotic cells have separated from each other and are lifted off the underlying basement membrane. Although the lesion is severe in the affected tubules, some adjacent tubules in the region are less damaged (L), thus a PCT score of 2. Bar = 50 [micro sign]m.