Figure 4. Midazolam potentiation of the [small gamma, Greek]-aminobutyric acid (GABA)-evoked (1 [micro sign]M) response. (A) Responses evoked by 1 [micro sign]M GABA were potentiated by increasing concentrations of midazolam (0.1 to 6 [micro sign]M). (B) Midazolam dose dependently potentiated GABA-evoked currents. The EC⁵⁰and Hill coefficient for midazolam potentiation were 0.102 +/− 0.034 [micro sign]M and 1.182 +/− 0.182, respectively (n = 9). High concentrations of midazolam increased the peak amplitude by 425 +/− 55%. The concentration of midazolam that caused the 300% effect level in the presence of 1 [micro sign]M GABA was 0.182 +/− 0.076 [micro sign]M for seven cells. The dashed line on the concentration-response curve indicates the dose that produced the specified effect level.