![Figure 5. The acute effect of halothane on the stimulated Ca2+level induced by U46619, platelet-activating factor, and thrombin. Experiments were done in the presence of 1 mM external CaCl2. Halothane (H, 1.6 mM) was added 30 s (arrow) after platelet stimulation with either 1 [micro sign]M U46619 (A) or 10 nM platelet-activating factor (PAF)(B) and 60 s after stimulation with 1 U/ml thrombin (C). This protocol allows the fast depletion of internal Ca2+stores, keeping an important contribution of the capacitative Ca2+influx. Control curves are labeled as C. Cytosolic Ca (2+) concentration is expressed as micromolar.](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/89/2/10.1097_00000542-199808000-00017/2/17ff5.png?Expires=1716438016&Signature=navtxyceAg-EJrXXI6oaIRkrmF1hZoMJRjCIqoPoSv7YHJTtV3oQ2k5VYletyjZg6hRZVi1O0T-xHbzvCmc3Lyg0cK7-JEA8B-WO1rnvDHAUDMnWMcWrM8GS~AqN2dIaqPp1niwabEEWBv99KZeVHn3y4t4D6-2wnYMzHvPlj8EDd-fqgi~nl9L33D5GttyRt-fCq6UGco898Hn25ALh7uW5fnyqlqZLvc3QPoEpRRvD1-xLxtQNT0wbp-YQkB2zXbGeGYzKszpgYwSwSrkxb24S6R10F-B6PECC6LJcLK~AT2bN3A01e5ia2cdL0VWVGcvIy0L3m9--srpVT7vlfg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 5. The acute effect of halothane on the stimulated Ca2+level induced by U46619, platelet-activating factor, and thrombin. Experiments were done in the presence of 1 mM external CaCl2. Halothane (H, 1.6 mM) was added 30 s (arrow) after platelet stimulation with either 1 [micro sign]M U46619 (A) or 10 nM platelet-activating factor (PAF)(B) and 60 s after stimulation with 1 U/ml thrombin (C). This protocol allows the fast depletion of internal Ca2+stores, keeping an important contribution of the capacitative Ca2+influx. Control curves are labeled as C. Cytosolic Ca (2+) concentration is expressed as micromolar.
