Figure 2. The effect of halothane on the Ca²⁺response of platelets to different stimulating agents. The effect of halothane on the amplitude of the peak Ca²⁺increase initiated by U46619 (1 [micro sign]M; A and C) or platelet-activating factor (PAF; 10 nM; B), or the Ca²⁺increase at 200 s triggered by thapsigargin (TG; 1 [micro sign]M; D). A significant effect (P < 0.05) compared with control (Ctr) was observed in U46619 and platelet-activating factor-stimulated cells, but halothane did not affect the Ca²⁺response when thapsigargin (TG) was used as stimulating agent. *P < 0.05. The analysis was based on n = 10 in A and n = 6 in B, C, and D.