Figure 3. Reversibility of local anesthetic inhibition of lysophosphatidate signaling. (A) With 15.5 mM bupivacaine. (B) With 42.7 mM lidocaine. Agonist was 10 sup -7 M throughout. The First bar shows control responses to lysophosphatidate; the second bar shows inhibition of lysophosphatidate signaling by the local anesthetic. Recovery experiments as shown in the third bar of the graphs were conducted by placing the oocyte in local anesthetic solution for 5 min and then washing for 5 min before lysophosphatidate treatment (average control response, 3.1 micro Coulomb; control group, n = 25; other groups, n = 5).