Figure 1. Typical recordings of glutamate release (arbitrary fluorescence units) evoked by cyanide anoxia (100 micro Meter NaCN) and isoflurane (approximately 1 minimum alveolar concentration) plus cyanide anoxia (100 micro Meter NaCN); study temperature was 37 degrees Celsius. Evoked release is calculated from the difference between the slope before and after exposure of the brain slice, either to isoflurane or to cyanide.