Figure 3. Effects of halothane (H) on the cytosolic Calcium2+concentration under InsP3-sensitive Calcium2+stores reloading conditions. (A) Effect of halothane on the capacitative Calcium2+increase initiated by addition of 1.8 mM Calcium2+to the bathing solution after depletion of internal Calcium2+pools through bradykinin (BK) receptor stimulation (10 nM) under Calcium2+-free conditions. Halothane (2 mM) was applied before the readmission of Calcium (2+) in the external medium and removed 50 s after the occurrence of a detectable Calcium2+rise. (B) InsP3-sensitive Calcium2+pools were first depleted by bath application of 10 nM bradykinin in external Calcium2+-free conditions and tested by a second brief application of bradykinin. The cells were then superfused for 50 s with a solution containing 1.8 mM Calcium2+with or without halothane to allow reloading of the Calcium2+pools. The Calcium2+content of the InsP3-sensitive Calcium2+pools was tested by perfusing with a Calcium2+-free external solution containing bradykinin. The light curve represents the experiment performed with 2 mM halothane during the reloading procedure.