Figure 1. Effects of halothane (H) and isoflurane (Iso) on the Calcium (2+) response evoked by 10 nM bradykinin (BK) in BAE cells. (A) Calcium (2+) response measured with the fluorescent Calcium2+indicator fura-2 in control conditions (dark curve) and in the presence of 2 mM halothane (light curve). The bathing medium was Earle's solution (1.8 mM Calcium2+) and the fluorescence signal represents the contribution of 10 to 20 cells. (B) Transient Calcium2+increase generated after the withdrawal of external halothane (0.8 mM). The external medium was Earle's solution (1.8 mM Calcium2+). (C) Effects of repetitive 50-s applications of 2 mM halothane on the Calcium2+response initiated after bradykinin receptor stimulation. (D) Effects of repetitive 50-s applications of 0.5 mM or 1 mM isoflurane on the Calcium2+response initiated after bradykinin receptor stimulation. Experimental conditions as described in A. Experiments were carried out at room temperature.