![Figure 5. Change in [Ca2+]inversus substance P in superfused P388D1cells. (A) Height of the peak response and (B) value during the plateau phase (Figure 4). Each data point is the mean plus/minus SEM from four to six separate measurements. Ordinates in Figure 5and Figure 6are the changes above resting [Ca2+]in. The average resting [Ca2+]inwas 83 plus/minus 12 nM (mean plus/minus SEM). The fitted curves are modified rectangular parabolas of the form:Equation 1where y = calcium response, X = substance P, and a, b, and c are empirical constants. For the curve in A, a = 1,040, b = 1.9, and c = 79; for B, a = 165, b = 1.9, and c = 79. In B, the scatter at substance P concentrations above 100 nM was large. All these data were used, however, for the curve fitting to arrive at a more accurate estimate of the level of [Ca2+]inproduced by saturating substance P.](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/82/1/10.1097_00000542-199501000-00021/2/21ff5.png?Expires=1716493252&Signature=SQTJDMkD1vfIC7VpaBJHztqCI3lf7OxnugdzK40d2K-g7z8q6yj20R5U1qt3oZpT8ImkSJIGwvsWDFHj21eMRBDN1JLpXOgEoAM1hd2UzHAQEV1WUjyDG9CeeV~mPlo-OiJ1dzTX0U26plRpMcRubFj9-Ic2VBcwnZuk87tjyem2uxJXOTbkmatMLW~qim8XjTuXHBlkKmKis22k655SsdY3fQewpqpvIoJI--A9MZ2LAQYBnFaeFoQQC-k~UVwuhmz5S7H0LxYfX8uGm-X16nB7ub0SBoHWRpEH0Btq6LQmmjY3F4AqIXQBI1NK7wOdAYFOiVODogke9rT8wXdwtg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 5. Change in [Ca2+]inversus substance P in superfused P388D1cells. (A) Height of the peak response and (B) value during the plateau phase (Figure 4). Each data point is the mean plus/minus SEM from four to six separate measurements. Ordinates in Figure 5and Figure 6are the changes above resting [Ca2+]in. The average resting [Ca2+]inwas 83 plus/minus 12 nM (mean plus/minus SEM). The fitted curves are modified rectangular parabolas of the form:Equation 1where y = calcium response, X = substance P, and a, b, and c are empirical constants. For the curve in A, a = 1,040, b = 1.9, and c = 79; for B, a = 165, b = 1.9, and c = 79. In B, the scatter at substance P concentrations above 100 nM was large. All these data were used, however, for the curve fitting to arrive at a more accurate estimate of the level of [Ca2+]inproduced by saturating substance P.
