Figure 2. Isoflurane inhibits the alpha4beta2 current in a dose-dependent manner. Concentration-response curve of the inhibition of alpha4beta2 currents by increasing concentrations of isoflurane. Peak currents were measured in response to increasing concentrations of isoflurane coapplied with 1 micro Meter acetylcholine. Data are fit to the Hill equation, and 95% confidence limits are expressed as dashed lines. The Hill coefficient is 1.3 and the median inhibitory concentration IC⁵⁰is 85 (+/- 7) micro Meter. P²is 34 (+/- 4.1). Data are expressed as means +/- SD.