Figure 4*. Effect of isoflurane on the total vasorelaxation response (filled bars) to bradykinin (A) or A23187 (B), and on the prostacyclin-mediated component (open bars) and the nitric oxide-mediated component (lined bars) of relaxation induced by these agonists. The nitric oxide-mediated and prostacyclin-mediated components of relaxation were calculated from areas above the agonist concentration-effect curves after inhibition with indomethacin and N^omega-nitro-L-arginine methyl ester, respectively (summarized in Figure 3and Figure 5). These areas are expressed as a percentage of the total vasorelaxation response (n = 5). Isoflurane attenuated (*P < 0.05) the total vasorelaxation response to both bradykinin and A23187 but had no effect on the individual vasorelaxant components mediated by either prostacyclin or nitric oxide.