Figure 4. Decrease in potency of pentobarbital induced by increasing cholesterol concentration. (A) Increased in IC⁵⁰: values were obtained from linear regression fits to the double reciprocal plots of the concentration-response data as shown above. Data were fitted with a rectangular hyperbola; B^max= 1,949 plus/minus 154 (SEM) micro Meter pentobarbital; EC⁵⁰- 1.9 plus/minus 1.4 (SEM)% cholesterol (weight/weight, corresponding to 3.5 mol%). (B) IC¹⁰values calculated for the same data as in Figure 4(A).