Fig. 1.
Inhibition of Axl with R428 did not worsen lung injury in murine ventilator-induced lung injury. Mice ventilated with injurious VT (20 ml/kg) exhibited decreased static compliance over time (*P < 0.05 vs. baseline) and compared to mice ventilated with protective VT (10 ml/kg). †P < 0.001 vs. 10 ml/kg (A, two-way repeated measures ANOVA for effects of group and time). Bronchoalveolar lavage (BAL) protein increased by up to 10%, but this finding did not reach significance (B, ANOVA on ranks). In mice ventilated with VT 20 ml/kg for 4 h, static compliance decreased over time (*P < 0.001 vs. baseline), but there was no difference in static compliance (C, two-way repeated measures ANOVA as above) or BAL protein (D, t test) between vehicle (Veh) and R428 groups. n = 4 per group. Myeloperoxidase (MPO) was increased in ventilated mice. †P < 0.001 versus nonventilated (NV; E, one-way ANOVA), but not significantly affected by R428 (F, t test). ns = nonsignificant.