Fig. 6.
Effects of dexmedetomidine and midazolam on A549 tumors in vivo. Mice with A549 tumors were treated daily for five days with saline, dexmedetomidine (0.5 mg/kg per day), or midazolam (2.5 mg/kg per day) via subcutaneous injection. No differences in animal weight (A) were detected, but midazolam significantly decreased tumor size (B). Representative photos taken of a mouse from each group before euthanasia; two of six mice treated with dexmedetomidine were observed to have red spots or scarring on tumor (C). Representative images of immunofluorescence staining of Ki67 (D) and cyclin D (E) from tumor sections showed an increase in both proteins in dexmedetomidine-treated mice and a decrease in midazolam-treated mice. Data are presented as mean ± SD. *P < 0.05 versus naïve control. n = 6 mice per group. Scale bar: ×10 magnification, 100 μm; ×20 magnification, 50 μm.