Fig. 4.
Neonatal exposure to isoflurane (ISO) or PDZ2WT peptide did not have an acute impact on the number of hippocampal postsynaptic densities. (A) Dorsal hippocampal region of a semi-thin section illustrating dentate gyrus (DG) subregion of interest (white box). (B) Representative ultrastructure images from postnatal day 21 mice exposed at postnatal day 7 to O2 (wild-type [WT] control [CON], 100% O2), WT isoflurane (1.5% isoflurane in O2), PDZ2MUT peptide (8 mg/kg inactive peptide), or PDZ2WT peptide (8 mg/kg active peptide); scale bar represents 500 nm. Asterisks showing some postsynaptic density (PSD) family members (not all are labeled). (C) Plots showing the median with interquartile (IQ) range number of PSDs. WT CON (n = 6) versus WT isoflurane (n = 5), P = 0.829; PDZ2MUT (n = 4) versus PDZ2WT (n = 4), P = 0.742. Data from individual animals are plotted and color-coded by sex (red, female; and blue, male). The data were analyzed with a Mann–Whitney test.