Fig. 2.
Western blot assays did not show any significant differences in apoptosis in our exposure paradigm. Postnatal day 7 mice were exposed for 4 h and harvested 2 h after cessation of exposure. (A) Western blot analysis for total caspase-3 revealed the presence of procaspase 3 but did not reveal any detectable cleaved caspase-3 in mice exposed to control (CON, O2), isoflurane (ISO, 1.5% isoflurane in O2), PDZ2MUT (8 mg/kg inactive peptide), or PDZ2WT (8 mg/kg active peptide). Negative (NC) and positive (PC) Jurkat control proteins were run to demonstrate sensitivity of the caspase-3 antibody. (B) Western blot analysis for poly-(adenosine diphosphate-ribose) polymerase (PARP) did not reveal significant differences between control (O2) and isoflurane (1.5% isoflurane in O2). (Left) Representative blot. (Right) Densitometry data are plotted as median, interquartile (IQ) range. CON (n = 6) versus isoflurane (n = 6), P = 0.818. The data were analyzed using Mann–Whitney test. Sex was not determined.