Fig. 3.
Remifentanil anesthesia further upregulates spinal protein kinase Mζ (PKMζ) activity, Kalirin-7 expression, and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptortrafficking after surgery. (A) Representative bands of Western blot for the levels of phosphorylated protein kinase Mζ, pPKMζ (Thr410, lower band, 55 kd), Kalirin-7, and total (t) and membrane (m) GluA1 and GluA2 protein at postoperative 48 h. (B–D) When compared with saline, incision and remifentanil upregulated the expression and phosphorylation of protein kinase Mζ (B), the level of Kalirin-7 (C), and the expression and trafficking of GluA1 (D). Moreover, the highest levels were noted in rats receiving incision and remifentanil treatment. (E) However, the expression and trafficking of GluA2 were unaltered. All the data are means ± SD (n = 4) and analyzed by one-way ANOVA with Bonferroni post hoc comparisons. #P < 0.05 versus group saline; *P < 0.05 versus group incision.

Remifentanil anesthesia further upregulates spinal protein kinase Mζ (PKMζ) activity, Kalirin-7 expression, and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptortrafficking after surgery. (A) Representative bands of Western blot for the levels of phosphorylated protein kinase Mζ, pPKMζ (Thr410, lower band, 55 kd), Kalirin-7, and total (t) and membrane (m) GluA1 and GluA2 protein at postoperative 48 h. (B–D) When compared with saline, incision and remifentanil upregulated the expression and phosphorylation of protein kinase Mζ (B), the level of Kalirin-7 (C), and the expression and trafficking of GluA1 (D). Moreover, the highest levels were noted in rats receiving incision and remifentanil treatment. (E) However, the expression and trafficking of GluA2 were unaltered. All the data are means ± SD (n = 4) and analyzed by one-way ANOVA with Bonferroni post hoc comparisons. #P < 0.05 versus group saline; *P < 0.05 versus group incision.

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