Exercise had no effects on postfracture neuropeptide expression in the sciatic nerve. Gene expression levels for substance P/tachykinin precursor 1 (TAC1, A), calcitonin-related polypeptide α (CALCA, B), and calcitonin-related polypeptide β (CALCB, C) were determined in the lumbar dorsal root ganglia by real-time polymerase chain reaction; substance P (D) and calcitonin gene-related peptide (E) protein levels in the sciatic nerve were determined by enzyme immunoassay. No changes in dorsal root ganglia substance P and calcitonin gene-related peptide gene expression or sciatic nerve protein expression were observed in the fracture limb at 7 weeks postfracture (FX + no wheel), compared to nonfractured control mice (control); 4 weeks of wheel running in fracture mice had no effect (FX + wheel). Values are means ± SD, n = 8 per cohort. One-way ANOVA with Bonferroni post hoc testing.