Expression of proinflammatory mediators after ventilation of mice with various inspiratory pressures (series_1). Mice were ventilated with 10 cm H2O (p10), 24 cm H2O (p24), 27 cm H2O (p27), and 30 cm H2O (p30) for 7 h. (A–D) Increased expression of proinflammatory response genes in mice. Relative mRNA levels were determined by qPCR in lung tissue. Transcript levels of interleukin 6 (Il6; p10: n = 6, p24: n = 5, p27: n = 5, p30: n = 6), tumor necrosis factor (Tnf; p10: n = 6, p24: n = 5, p27: n = 5, p30: n = 6), chemokine (C-X-C motif) ligand 1 (Cxcl1; p10: n = 6, p24: n = 5, p27: n = 5, p30: n = 6), and C-X-C motif ligand 10 (Cxcl10; p10: n = 5, p24: n = 5, p27: n = 5, p30: n = 6) were normalized to TATA box binding protein (Tbp). Gene expression is depicted as fold induction relative to unventilated controls. (E–H) Increased release of cytokines in mice. Cytokine levels were determined by enzyme-linked immunosorbent assays in bronchoalveolar lavage fluid (BALF). Cytokine quantity of IL6 (p10: n = 5, p24: n = 5, p27: n = 4, p30: n = 6), TNF (p10: n = 4, p24: n = 5, p27: n = 4, p30: n = 5), chemokine (C-X-C motif) ligand 1 (CXCL1) (p10: n = 5, p24: n = 4, p27: n = 4, p30: n = 5), and C-X-C motif ligand 10 (CXCL10) (p10: n = 5, p24: n = 4, p27: n = 4, p30: n = 6) were analyzed. (A–H), Data are shown as mean plus SD. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 in comparison to the 24 cm H2O group. n.s. = not significant.
