Figure 3. Effects of ketamine and pentobarbital on the norepinephrine (NET)(A), dopamine (DAT)(B), and serotonin (SERT)(C) transporter cells. Human embryonic kidney (HEK)-NET, HEK-DAT and HEK-SERT cells were incubated with transport buffers containing 192.4 nM³H-norepinephrine, 67.2 nM³H-dopamine, and 76.1 nM³H-serotonin, respectively, and various concentrations of ketamine (filled diamond) or pentobarbital (open triangle). Uptake was determined after 6 min. Each point represents the mean +/- SD of three experiments performed in quadruplicate. In all cases, the Hill coefficient obtained from curve fitting was 1.