Fig. 1.
Disruption of transient receptor potential melastatin 2 (TRPM2) aggravated the outcome of Escherichia coli sepsis and decreased bacterial elimination in peritoneal macrophages. (A) Trpm2+/+ and Trpm2−/− mice were intraperitoneally challenged with 2 × 107 colony-forming units (CFU) of E. coli, and survival was monitored for 72 h. Data consist of two independent experiments (n = 21 per group) and were analyzed by the Kaplan–Meier log-rank test. (B) Peritoneal bacterial burdens were determined in Trpm2+/+ and Trpm2−/− mice after intraperitoneal injection of E. coli (2 × 107 CFU; n = 5 per group). Horizontal bars represent median values, and dots represent individual mice. Student’s t test was used to compare differences between the two independent groups. (C) Peritoneal macrophages derived from Trpm2+/+ and Trpm2−/− mice were exposed to E. coli, and bacterial killing was assessed by gentamycin assay. Data were compared using Student’s t test (n = 4). (D) Uptake of E. coli by Trpm2+/+ and Trpm2−/− peritoneal macrophages were assessed (multiplicity of infection [MOI] = 10; n = 4 per group). Data were analyzed using Student’s t test. (E) Phagocytic capacity of peritoneal macrophages from Trpm2+/+ and Trpm2−/− mice was evaluated using microspheres by fluorescence microscopy (MOI = 10). At least 100 macrophages were counted for each experiment (n = 3). Data were analyzed using Student’s t test. *P < 0.05; **P < 0.01. PLF = peritoneal lavage fluid.