Fig. 1.
Intensity of blockade at 15-min postinjection for hotplate, von Frey testing, and extensor postural thrust in the injected limb (A–C) and the contralateral limb (D–F). Horizontal lines represent median value, boxes represent interquartile range, and whiskers represent minimum–maximum values. For the injected limb, Kruskal–Wallis tests showed a significant difference in intensity between doses for Neosaxitoxin-saline (NeoSTX-Saline) (von Frey P < 0.001; hotplate P < 0.001; and extensor postural thrust P < 0.001). For Neosaxitoxin-Bupivacaine (NeoSTX-Bup), only extensor postural thrust test was significant (P = 0.002). For the contralateral limb, Kruskal–Wallis tests showed a significant difference in intensity between doses for NeoSTX-Saline (hotplate P < 0.001) and for NeoSTX-Bup (hotplate P < 0.001 and extensor postural thrust P < 0.001) tests. Comparisons have been presented for NeoSTX-Saline versus NeoSTX-Bup using Mann–Whitney test at each Neosaxitoxin (NeoSTX) dose. All bupivacaine-containing formulations produced dense block in all animals at 15 min.