Fig. 4.
The effects of GAL-021 on the peak change in minute volume () in urethane-anesthetized rats with intact (sham operated) and bilaterally transected carotid sinus nerves (CSN). CSN transection (Tx) abolished the ventilatory response to acute hypoxia (Fio2 = 0.12 during 3 min), confirming the functional completeness of the carotid body denervation. There was no effect on breathing of administering vehicle (saline) before or after carotid body denervation. The effects of GAL-021 (0.3 mg/kg IV bolus) on breathing were markedly diminished in rats with carotid body denervation compared with sham-operated controls. *Different from sham-operated rats (two-way ANOVA with Sidak multiple comparisons tests; P < 0.05). Values are means ± SD, n = 6.