Fig. 3.
![1H NMR spectral analysis (600 MHz, room temperature). Head-to-head competition between sugammadex and calabadion 2 to assess their relative binding affinity toward rocuronium. Chemical shift (in parts per million [ppm]) is shown on the x-axes and signal intensity on the y-axes. (A) Rocuronium 2 mM, a and b are the resonances for the CH3 groups of free rocuronium. (B) An equimolar solution of calabadion 2 and rocuronium (2 mM). a′ and b′ are resonances for the bound CH3 groups of calabadion 2•rocuronium. (C) An equimolar solution of sugammadex and rocuronium (2 mM), a″ and b″ are resonances for the bound CH3 groups of sugammadex•rocuronium. (D) A solution containing 186.8 μM calabadion 2, 199.8 μM rocuronium, and 2399.7 μM sugammadex.](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/123/6/10.1097_aln.0000000000000868/2/25ff03.png?Expires=1716635024&Signature=hBEg8TSaUuG5bm2NBjDbfxcE~TVjEmXCTJjTpQW8ctpKuZTcooQ-BXsOWUey2HQBt4aYuu~b1ep9Ds06GwVHoR4Rd6VNmBKkEnQcBFDyBVHm0YEWzW7Y0Cutt79SY~m6nYUM6IqPd1FeQn4aEluNDSQFjt5YR0~X3VTst6sPmcUhmM8gB5Ue7jQav4s-O~pLrZwVryTpzpmOXvhVOflWBBhIMnQWyJtOwd5p12dMw8blFT5~nVgVokakG5kRiCrY5uCJVn3WzaRnepszVaM-QcxwcFxtJLJMXpBNf-juSo3P~uI5bwxpQgYc18Haec-sLn9Jpk6lIjNQ9LH2gn9vAA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
1H NMR spectral analysis (600 MHz, room temperature). Head-to-head competition between sugammadex and calabadion 2 to assess their relative binding affinity toward rocuronium. Chemical shift (in parts per million [ppm]) is shown on the x-axes and signal intensity on the y-axes. (A) Rocuronium 2 mM, a and b are the resonances for the CH3 groups of free rocuronium. (B) An equimolar solution of calabadion 2 and rocuronium (2 mM). a′ and b′ are resonances for the bound CH3 groups of calabadion 2•rocuronium. (C) An equimolar solution of sugammadex and rocuronium (2 mM), a″ and b″ are resonances for the bound CH3 groups of sugammadex•rocuronium. (D) A solution containing 186.8 μM calabadion 2, 199.8 μM rocuronium, and 2399.7 μM sugammadex.
