Decreased infiltration of F4/80⁺CD206⁺ M2 macrophages was restored by coadministration of rosiglitazone (rosi) with resolvin D1 (RvD1) in db/db mice. (A) Infiltration of F4/80⁺iNOS⁺ M1 macrophages and F4/80⁺CD206⁺ M2 macrophages was evaluated on days 2 and 7. (B) The number of F4/80⁺iNOS⁺ and total F4/80⁺ macrophages. Two-way ANOVA (F4/80⁺iNOS⁺: interaction factor: F_5,84 = 0.3126, P = 0.9041; total F4/80⁺: interaction factor: F_5,84 = 0.7995, P = 0.5532). (C) The number of F4/80⁺CD206⁺ and total F4/80⁺ macrophages. Two-way ANOVA (F4/80⁺CD206⁺: interaction factor: F_5,84 = 1.132, P = 0.4350; total F4/80⁺: interaction factor: F_5,84 = 0.8349, P = 0.3498). The results are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Green = F4/80; red = iNOS or CD206; blue = 4’,6-diamidino-2-phenylindole (DAPI); Scale bar = 100 μm. iNOS = inducible nitric oxide synthase.