Fig. 5.
Apoptotic TdT-mediated dUTP nick-end labeling (TUNEL)+ cells were markedly decreased by rosiglitazone (rosi) coadministered with resolvin D1 (RvD1) in db/db mice at the incision site. (A) TUNEL+CD68+ DAPI+, TUNEL+DAPI+, and CD68+DAPI+ cells at the incised sites. (B) The number of TUNEL+CD68+ DAPI+ per area at the incision sites. Two-way ANOVA (interaction factor: F7,96 = 2.806, P = 0.0106) by separate one-way ANOVA with Tukey post hoc test for comparison at each time (day 2: F7,48 = 7.587, P < 0.0001; day 7: F7, 48 = 2.891, P = 0.0133). (C) The number of TUNEL+DAPI+ cells per area at the incision sites. Two-way ANOVA (interaction factor: F7,96 = 0.8077, P = 0.5829) followed by Tukey post hoc test for comparing differences among groups. (D) The number of CD68+ DAPI+ cells per area at the incision sites. Two-way ANOVA (interaction factor: F7,96 = 0.6218, P = 0.7368). The results are presented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. Green = TUNEL; red = CD68; blue = DAPI. Scale bar, 100 μm. DAPI = 4’,6-diamidino-2-phenylindole.