Fig. 4.
Neuropathic injury up-regulates colocalized phosphorylated histone deacetylase (pHDAC4) and t14-3-3β expressions in the ipsilateral dorsal horn. (A) Immunohistochemical images showing that spinal nerve ligation (SNL) enhanced the immunoreactivity of pHDAC4 (I and IV, red) and total 14-3-3β (t14-3-3β; II and V, green) in the ipsilateral (IPSI) compared with the contralateral (CONTRA) dorsal horn (I, VII and II, VIII, respectively) of the spinal sections obtained at day 7 after operation (7D). Overlay images and the count of double-labeled neurons showing the enhanced pHDAC4 fluorescence (IV) costained with t14-3-3β immunoreactivity (V) in the ipsilateral (III, VI, and VIa, yellow), but not in the contralateral (III, IX, and IXa), dorsal horn. Scale bar = 50 μm, thickness = 50 μm. Each of these immunofluorescence images was replicated in seven sample preparations with similar results each time. (B) Statistical analysis showing SNL enhanced the count of pHDAC4-positive, t14-3-3β-positive, and double-labeled neurons in the ipsilateral dorsal horn of the spinal sections obtained at day 7 after operation (**P < 0.01 compared with the CONTRA, n = 7).