Fig. 1.
Western blotting analysis of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels expression in dorsal root ganglia (DRG) and sciatic nerves. (A) Both HCN1 and HCN2 channel protein with expected molecular weight (approximately 100 kDa) were detected in DRG and/or sciatic nerves from wild-type mice (n = 4), and no HCN1 protein (with expected molecular weight) was found in DRG or sciatic nerves from HCN1−/− mice (n = 3). (B) Quantitative analysis was determined by the ratio between HCN1 or HCN2 and β-actin immunoreactivity. KO = HCN1−/− knockout; WT = wild type.