Fig. 2.
The effects of the sevoflurane anesthesia on Tau phosphorylation are not dependent on the time of harvest. (A) Anesthesia with 3% sevoflurane 2 h daily for 1 day in P6 wild-type mice does not increase the levels of AT8(Tau-PS202 and Tau-PT205) and total Tau in the hippocampus of the mice (harvested on P6) as compared with the control condition. There is no significant difference in β-actin levels in the hippocampus of the mice between the sevoflurane anesthesia and the control condition. (B) Quantification of the Western blot shows that the sevoflurane anesthesia does not increase the ratio of AT8(Tau-PS202 and Tau-PT205) to total Tau in the hippocampus of the mice as compared with the control condition. (C) Anesthesia with 3% sevoflurane 2 h daily for 3 days in P6 mice increases the levels of AT8(Tau-PS202 and Tau-PT205), but not total Tau, in the hippocampus of the mice (harvested on P10) as compared with the control condition. There is no significant difference in β-actin levels in the hippocampus of the mice between the sevoflurane anesthesia and the control condition. (D) Quantification of the Western blot shows that the sevoflurane anesthesia increases the ratio of AT8(Tau-PS202 and Tau-PT205) to total Tau in the hippocampus of the mice as compared with the control condition. (E) Anesthesia with 3% sevoflurane 2 h daily for 3 days in P6 wild-type mice does not increase the levels of Tau-PS262 in the hippocampus of the mice (harvested on P8) as compared with the control condition. There is no significant difference in β-actin levels in the hippocampus of the mice between the sevoflurane anesthesia and control condition. (F) Quantification of the Western blot shows that the sevoflurane anesthesia does not increase Tau-PS262 levels in the hippocampus of the mice as compared with the control condition. AT8 = antibody to detect Tau-PS202 and Tau-PT205; N.S. = nonsignificant; P = phosphorylated; Tau-PS202 = Tau phosphorylated at serine-202; Tau-PS262 = Tau phosphorylated at serine 262; Tau-PT205 = Tau phosphorylated at threonine 205. **P < 0.01; N = 6 in each group.

The effects of the sevoflurane anesthesia on Tau phosphorylation are not dependent on the time of harvest. (A) Anesthesia with 3% sevoflurane 2 h daily for 1 day in P6 wild-type mice does not increase the levels of AT8(Tau-PS202 and Tau-PT205) and total Tau in the hippocampus of the mice (harvested on P6) as compared with the control condition. There is no significant difference in β-actin levels in the hippocampus of the mice between the sevoflurane anesthesia and the control condition. (B) Quantification of the Western blot shows that the sevoflurane anesthesia does not increase the ratio of AT8(Tau-PS202 and Tau-PT205) to total Tau in the hippocampus of the mice as compared with the control condition. (C) Anesthesia with 3% sevoflurane 2 h daily for 3 days in P6 mice increases the levels of AT8(Tau-PS202 and Tau-PT205), but not total Tau, in the hippocampus of the mice (harvested on P10) as compared with the control condition. There is no significant difference in β-actin levels in the hippocampus of the mice between the sevoflurane anesthesia and the control condition. (D) Quantification of the Western blot shows that the sevoflurane anesthesia increases the ratio of AT8(Tau-PS202 and Tau-PT205) to total Tau in the hippocampus of the mice as compared with the control condition. (E) Anesthesia with 3% sevoflurane 2 h daily for 3 days in P6 wild-type mice does not increase the levels of Tau-PS262 in the hippocampus of the mice (harvested on P8) as compared with the control condition. There is no significant difference in β-actin levels in the hippocampus of the mice between the sevoflurane anesthesia and control condition. (F) Quantification of the Western blot shows that the sevoflurane anesthesia does not increase Tau-PS262 levels in the hippocampus of the mice as compared with the control condition. AT8 = antibody to detect Tau-PS202 and Tau-PT205; N.S. = nonsignificant; P = phosphorylated; Tau-PS202 = Tau phosphorylated at serine-202; Tau-PS262 = Tau phosphorylated at serine 262; Tau-PT205 = Tau phosphorylated at threonine 205. **P < 0.01; N = 6 in each group.

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