Fig. 3. Time course and dose–response of isoflurane postconditioning. Corticostriatal slices were postconditioned with 2% isoflurane for various durations after a 15-min oxygen–glucose deprivation (OGD) (A ) or with various concentrations of isoflurane for 30 min after a 15-min OGD (B ). Isoflurane was applied at the onset of simulated reperfusion after the OGD. Cell injury was quantified by 2,3,5-triphenyltetrazolium chloride (TTC) conversion 2 h after the OGD. Results are mean ± SD (n = 11 for A and n = 12 for B ). Statistical analysis was performed by one-way repeated-measures analysis of variance on ranks followed by the Dunn method. * P < 0.05 compared with OGD only.