Fig. 2. Representative sections of corticostriatal slices stained with hematoxylin and eosin. The oxygen–glucose deprivation (OGD) was for 15 min at 37°C. Injured cells are presented with one of the following characteristics: cell swelling; vacuolization (commonly associated with light-staining cytoplasm); or presence of shrunken, darkened nuclei.  Arrows in the OGD panel indicated neurons with condensed nuclei. The  bar chart presents the quantification data. Results are mean ± SD (n = 15). Statistical analysis was performed by one-way repeated-measures analysis of variance followed by the Dunn method. *  P < 0.05 compared with control. ˆ  P < 0.05 compared with OGD only. Con = control; Iso = 2% isoflurane applied for 30 min at the onset of simulated reperfusion after the OGD.  Scale bar : 20 μm.