Fig. 3. Representative autoradiograph of RNase protection assay showing the time course of cytokine gene expression in mononuclear blood cells. A total of 10 μg RNA was used in each lane. CPB = cardiopulmonary bypass; CO = carbon monoxide; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; IL = interleukin; RANTES = regulated upon activation, normal T-cell expressed, and secreted; Y = yeast transfer RNA.