Fig. 3. Effects of isoflurane pretreatment on inducible nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) plus interferon γ (IFN-γ). Rat NR8383 macrophages were pretreated with or without various concentrations of isoflurane (Iso). They were then exposed to 100 ng/ml LPS plus 50 U/ml IFN-γ for 24 h at 30 min after the isoflurane pretreatment. These cells and the cells that were not exposed to isoflurane and LPS plus IFN-γ (normal culture) were harvested for Western analysis. Results are mean ± SD (n = 9–25). *  P < 0.05 compared with the corresponding cells exposed to LPS plus IFN-γ only. 

Fig. 3. Effects of isoflurane pretreatment on inducible nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) plus interferon γ (IFN-γ). Rat NR8383 macrophages were pretreated with or without various concentrations of isoflurane (Iso). They were then exposed to 100 ng/ml LPS plus 50 U/ml IFN-γ for 24 h at 30 min after the isoflurane pretreatment. These cells and the cells that were not exposed to isoflurane and LPS plus IFN-γ (normal culture) were harvested for Western analysis. Results are mean ± SD (n = 9–25). *  P < 0.05 compared with the corresponding cells exposed to LPS plus IFN-γ only. 

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