Fig. 5. Withdrawal thresholds ipsilateral to sham or L5 nerve transection surgery before (baseline [BL]) and 1 and 4 days (D1 and D4, respectively) after surgery, in L5 nerve transection animals after intrathecal administration of vehicle (VEH), JWH015 (JWH), CP55940 (CP), JWH015 plus AM630 (AM2), or AM281 (AM1), and in sham animals after vehicle (SHAM VEH) or JWH015 (SHAM JWH 10 μg). ( A ) Withdrawal thresholds to von Frey stimulation ipsilateral to sham or L5 nerve transection surgery before and 1 and 4 days after surgery and 15 min, 30 min, 1 h, and 2 h after first and second injection (1st Inj. and 2nd Inj., respectively) of vehicle (n = 7), CP55940 (10 μg, n = 6), or 0.4, 1, 2, or 10 μg JWH015 (n = 5, 5, 7, and 10, respectively) for L5 nerve transection animals, and JWH015 (10 μg, n = 5) or vehicle (n = 11) in sham animals. Over time, values versus D4 after surgery significantly differ by Friedman test; * P < 0.05 versus D4 after surgery by Friedman test followed by Wilcoxon test. Groups significantly differ by Kruskal–Wallis test; P < 0.05, Kruskal–Wallis test followed by Mann–Whitney U test, was found in L5 nerve transection with CP55940 versus L5 nerve transection with vehicle at 15 min, 30 min, and 1 h after the second injection; L5 nerve transection with 1 μg JWH015 versus L5 nerve transection with vehicle at 15 min after the second injection; L5 nerve transection with 2 μg JWH015 versus L5 nerve transection with vehicle at 2 h after the second injection; L5 nerve transection with 10 μg JWH015 versus L5 nerve transection with vehicle at all times tested after the second injection; sham with vehicle or 10 μg JWH015 versus L5 nerve transection with vehicle at all times tested after surgery. ( B ) Withdrawal thresholds to von Frey stimulation ipsilateral to L5 nerve transection surgery before and 4 days after surgery (After Surg) and 1 and 2 h after first and second injection of 10 μg JWH015 alone or plus AM630 (10 μg, n = 7) or AM281 (10 μg, n = 7). Groups significantly differ by Kruskal–Wallis test; * P < 0.05 compared with JWH015 plus AM630 group and + P < 0.05 compared with vehicle group by Kruskal–Wallis test followed by Mann–Whitney U test.