Fig. 4.
3′3-Diaminobenzidine HCl immunohistochemistry for nerve growth factor (NGF) in plantar skin. (A) NGF immunohistochemistry in sham-operated skin. (B) NGF (arrows) in dermis adjacent to the incision (dashed line) on postoperative day (POD) 1. (C) A higher magnification of the rectangular area inB. NGF is indicated by thearrows. (D) NGF in incised plantar skin was not evident on POD 7. Thearrowindicates the incision.Scale bars: 100 μm forAandB; 50 μm forC; 200 μm forD. Der = dermis; Epi = epidermis.